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Enhancement Assay (Figure 4): The next buffers had been used: Coating buffer: 0.1 M Na2CO3, pH 9.5; blocking buffer: 1% BSA in 0.1 M phosphate buffer pH 7.2; sample buffer: 0.1% BSA and 0.01% Tween 20 in 0.1 M phosphate buffer pH 7.2; washing buffer: phosphate buffer pH 7.2 containing 0.01% Tween 20. The wells … Read more

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